protocol

The Clough Lab

Protocols

RNA Isolation and Gel Verification

RNA Isolation Using Trizol and PLG-heavy Tubes (.doc, 32 k)
RNA Isolation (Vodkin Method) (.doc, 32 k)
RNA Isolation (Damla Bilgin Method) (.pdf, 120 k)
Quality checking by running gel on Agilent BioAnalyzer (.doc, 13.8 MB)

Microarray Protocols
- Indirect Labelling with Amino Allyl dUTP, July 2008 (.doc, 230 k)
- 5X dNTP mix Nov 6, 2009 (.doc, 34 k)
- Direct Labelling RNA (.rtf, 52 k)
- Direct Labelling DNA (.doc, 31 k)
- Blocking Slides SDS washing (.doc, 36 k)

Inoculations
- Pseudomas Syringae Vacuum Infiltration
- Sclerotinia stem cut inoculation

Transformation
- Arabidopsis- Floral Dip Protocol
- Soybean Cotyledonary Node- Paula Olhofts Transformation Protocol (.doc, 48 k)

Agrobacterium protocols
- Agrobacterium Plasmid Prep (.doc, 39 k)
- Electroporation Competent Cells (.doc, 26 k)
- Electroporation (.doc, 23 k)
- Triparental Matings (.doc, 34 k)

Tissue Culture Protocols

GUS staining (.doc, 28 k)
Seed Sterilization (.doc, 28 k)
Media
- Germination
  - Germination Medium (.doc, 43k)
- Shoots
  - Modified Shoot Induction (.doc, 56 k)
  Rooting
  - Rooting Medium (.doc, 46 k)
  Other Media
  - C5 Medium (.doc, 60 k)
    D1 Medium (.doc, 48 k)
    Soybean Rescue Medium (.doc, 64 k)
  - Co-cultivation Medium (.doc, 52 k)
- Stock Solutions
  - MS Major Stock (.doc, 36 k)
    MS Minor Stock (.doc, 40 k)
    MSIII Iron Stock (.doc, 36 k)
    B5 Major(.doc, 40 k)
    B5 Minor Stock (.doc, 36 k)
  - B5 Vitamin Stock (.doc, 32 k)

This page last updated: November 12, 2009
Designed by: Marisol Benitez and Jason Bant